84-4352-89　EP300 (KAT3B, p300, CREBBP/EP300 Inhibitory Protein 1, Cyclic AMP Responsive Enhancer Binding Protein, E1A Associated Protein p300, E1A Binding Protein p300, E1A-associated Protein p300, EP300: E1A Binding Protein p300, EP300_HUMAN, Histone Acetyltransferase p300, p300 HAT, RB and P300 Binding Protein EID 1, Retinoblastoma Protein Associated Protein, RSTS2) 100ul　327172

特徴

• Functions as histone acetyltransferase and regulates transcription via chromatin remodeling.
• Acetylates all four core histones in nucleosomes.
• Histone acetylation gives an epigenetic tag for transcriptional activation.
• Mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein.
• Mediates acetylation of histone H3 at 'Lys-122' (H3K122ac), a modification that localizes at the surface of the histone octamer and stimulates transcription, possibly by promoting nucleosome instability.
• Mediates acetylation of histone H3 at 'Lys-27' (H3K27ac).
• Also functions as acetyltransferase for nonhistone targets.
• Acetylates 'Lys-131' of ALX1 and acts as its coactivator in the presence of CREBBP.
• Acetylates SIRT2 and is proposed to indirectly increase the transcriptional activity of TP53 through acetylation and subsequent attenuation of SIRT2 deacetylase function.
• Acetylates HDAC1 leading to its inactivation and modulation of transcription.
• Acts as a TFAP2A-mediated transcriptional coactivator in presence of CITED2.
• Plays a role as a coactivator of NEUROD1-dependent transcription of the secretin and p21 genes and controls terminal differentiation of cells in the intestinal epithelium.
• Promotes cardiac myocyte enlargement.
• Can also mediate transcriptional repression.
• Binds to and may be involved in the transforming capacity of the adenovirus E1A protein.
• In case of HIV-1 infection, it is recruited by the viral protein Tat.
• Regulates Tat's transactivating activity and may help inducing chromatin remodeling of proviral genes.
• Acetylates FOXO1 and enhances its transcriptional activity.
• Acetylates BCL6 wich disrupts its ability to recruit histone deacetylases and hinders its transcriptional repressor activity.
• Participates in CLOCK or NPAS2-regulated rhythmic gene transcription; exhibits a circadian association with CLOCK or NPAS2, correlating with increase in PER1/2 mRNA and histone H3 acetylation on the PER1/2 promoter.Wuchao Yuan, J.
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• Applications:Suitable for use in Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:3000Immunohistochemistry: 1:50-1:100Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.