4E-BP1 encodes one member of a family of translation repressor proteins.
The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs.
Interaction of this protein with eIF4E inhibits complex assembly and represses translation.
This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.
Gingras AC, et al.
(1998) Genes Dev 12(4): 502-513.
Brugarolas J, et al.
(2004) Genes Dev 18(23): 2893-2904.
Kumar V, et al.
(2000) EMBO J 19(5): 1087-1097.
Moody CA, et al.
(2005) J Virol 79(9): 5499-5506.
Burnett PE, et al.
(1998) Proc Natl Acad Sci U S A 95(4): 1432-1437.
Applications:Suitable for use in Immunofluorescence and Western Blot.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 1:100-1:200Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
Specificity：Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：IF WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Peptide corresponding to the sequence around aa43-47 (S-T-T-P-G) derived from Human 4E-BP1.
