This gene is a target gene of the transcription factor tumor protein p53.
The encoded protein is a nuclear phosphoprotein that binds and inhibits transactivation by tumor protein p53, as part of an autoregulatory negative feedback loop.
Overexpression of this gene can result in excessive inactivation of tumor protein p53, diminishing its tumor suppressor function.
This protein has E3 ubiquitin ligase activity, which targets tumor protein p53 for proteasomal degradation.
This protein also affects the cell cycle, apoptosis, and tumorigenesis through interactions with other proteins, including retinoblastoma 1 and ribosomal protein L5.
More than 40 different alternatively spliced transcript variants have been isolated from both tumor and normal tissues Haupt, Y.
et al.
(1997) Nature 387, 296-299.
Zhou, B.
P.
et al.
(2001) Nat.
Cell Biol.
3, 973-981.
Grossman, S.
R.
et al.
(1998) Mol.
Cell 2, 405-415.
Mayo, L.D.
and Donner, D.B.
(2001) Proc.
Natl.
Acad.
Sci.
USA 98, 11598-11603.
Applications:Suitable for use in Immunofluorescence and Western Blot.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 1:100-1:200Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
Specificity：Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：IF WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Peptide corresponding to the sequence around aa164-168 (A-I-S-E-T) derived from Human MDM2.
