The protein encoded by Cyclin B1 is a regulatory protein involved in mitosis.
The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF).
Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase.
The different transcripts result from the use of alternate transcription initiation sites.
Norbury, C.
and Nurse, P.
(1992) Annu.
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Atherton-Fessler, S.
et al.
(1993) Mol.
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13, 1675-1685.
Galaktionov, K.
et al.
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9, 1046-1058.
Applications:Suitable for use in Immunofluorescence and Western Blot.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 1:100-1:200Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
Specificity：Human
Isotype：IgG
Calc Applications Abbrev：IF WB
Calc Crossreactivity：Hu
Immunogen：Peptide corresponding to the sequence around aa145-149 (A-F-S-D-V) derived from Human Cyclin B1.
