eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions.
It is composed of at least EIF4A, EIF4E and EIF4G1/EIF4G3.
Interacts with eIF3, mutually exclusive with EIF4A1 or EIFA2, EIF4E and through its N-terminus with PAPBC1.
Interacts through its C-terminus with the serine/threonine kinases MKNK1, and with MKNK2.
Appears to act as a scaffold protein, holding these enzymes in place to phosphorylate EIF4E.
Non-phosphorylated EIF4EBP1 competes with EIF4G1/EIF4G3 to interact with EIF4E; insulin stimulated MAP-kinase (MAPK1 and MAPK3) phosphorylation of EIF4EBP1 causes dissociation of the complex allowing EIF4G1/EIF4G3 to bind and consequent initiation of translation.
EIF4G1/EIF4G3 interacts with PABPC1 to bring about circularization of the mRNA.
Rapamycin can attenuate insulin stimulation mediated by FKBPs.
Interacts with EIF4E3.
Interacts with MIF4GD.
Interacts with rotavirus A NSP3; in this interaction, NSP3 takes the place of PABPC1 thereby inducing shutoff of host protein synthesis De Gregorio, E.
et al.
(1998) RNA 4, 828-836.
Ohlmann, T.
et al.
(1996) EMBO J.
15, 1371-1382.
Borman, A.M.
and Kean, K.M.
(1997) Virology 237, 129-136.
Gradi, A.
et al.
(1998) Mol Cell Biol 18, 334-42.
Applications:Suitable for use in Immunofluorescence and Western Blot.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 1:100-1:200Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
Specificity：Human
Isotype：IgG
Calc Applications Abbrev：IF WB
Calc Crossreactivity：Hu
Immunogen：Peptide corresponding to the sequence around aa1230-1234 (P-V-S-P-L) derived from Human eIF4G.
