84-4351-69　mTOR (Mammalian Target of Rapamycin, FK506 Binding Protein 12 Rapamycin Complex Associated Protein 1, FK506 Binding Protein 12-Rapamycin Associated Protein 1, FRAP1, FK506 Binding Protein12-Rapamycin Associated Protein 2, FRAP2, FKBP12 Rapamycin Complex Associated Protein, FRAP, RAFT1, Rapamycin Target Protein, RAPT1) 100ul　324596

特徴

• Kinase subunit of both mTORC1 and mTORC2, which regulate cell growth and survival in response to nutrient and hormonal signals.
• mTORC1 is activated in response to growth factors or amino-acids.
• Amino-acid-signaling to mTORC1 is mediated by Rag GTPases, which cause amino-acid-induced relocalization of mTOR within the endomembrane system.
• Growth factor-stimulated mTORC1 activation involves AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1.
• Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis.
• mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E).
• mTORC1 phosphorylates and activates S6K1 at 'Thr-421', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation.
• mTORC2 is also activated by growth factors, but seems to be nutrient-insensitive.
• mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors.
• mTORC2 promotes the serum-induced formation of stress-fibers or F-actin.
• mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation.
• mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'.
• mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'.
• Albanell J,et al.(2007)Clin Transl Oncol.9(8):484-93.
• Huang JJ,et al.
• Applications:Suitable for use in Immunofluorescence and Western Blot.
• Other applications not tested.
• Recommended Dilution:Immunofluorescence: 1:100-1:200Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.