Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase.
It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase.
Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia.
This is a catalytic subunit.
Hurley RL, et al.
(2005) J Biol Chem.
Aug 12; 280(32): 29060-29066 Woods A, et al.
(2003) Curr Biol.
Nov 11; 13(22): 2004-2008 Nielsen JN, et al.
(2003) J Appl Physiol.
Feb; 94(2): 631-641 Da Silva Xavier G, et al.
(2000) Proc Natl Acad Sci U S A.
Apr 11; 97(8): 4023-4028.
Applications:Suitable for use in Immunofluorescence and Western Blot.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 1:100-1:200Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
Specificity：Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：IF WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Peptide corresponding to the sequence around aa172-176/170-174 (L-R-T-S-C) derived from Human AMPKa1/AMPKa2.
