FKHR belongs to the forkhead family of transcription factors, which are characterized by a distinct forkhead domain.
It may play a role in myogenic growth and differentiation.
The mammalian DAF-16-like transcription factors, FKHR, FKHRL1, and AFX, function as key regulators of insulin signaling, cell cycle progression, and apoptosis downstream of phosphoinositide 3-kinase.
Gene activation through binding to insulin response sequences has been essential for mediating these functions.
D-type Cyclins (in Class III) is required for FKHR mediated inhibition of cell cycle progression and transformation.
FKHR gene is mapped to chromosome 13q14 Gan L, et al.
(2005) J Neurochem; 93(5): 1209-19.
Smith WW, et al.
(2005) J Cell Biol; 169(2): 331-9.
Di Maira G, et al.
(2005) Cell Death Differ; 12(6): 668-77.
Horn S, et al.
(2004) Leukemia; 18(11): 1839-49.
Zhao X, et al.
(2004) Biochem J 4; 378(Pt 3): 839-49.
Applications:Suitable for use in Immunofluorescence and Western Blot.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 1:100-1:200Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
Specificity：Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：IF WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Peptide corresponding to the sequence around aa254-258 (A-A-S-M-D) derived from Human FKHR/FOXO1A.
