This kinase is activated by mitogenic and environmental stress, and participates in the MAPK-mediated signaling cascade.
It phosphorylates and thus activates p38.
This kinase can be activated by insulin, and is necessary for the expression of glucose transporter.
Expression of Ras oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of p38, and confers oncogenic transformation of primary cells.
The inhibition of this kinase is involved in the pathogenesis of Yersina pseudotuberculosis.
Wang W, et al.
(2002) Mol Cell Biol ; 22(10): 3389-403.
Raingeaud J, et al.
(1996) Mol Cell Biol; 16(3): 1247-55.
Applications:Suitable for use in Immunofluorescence and Western Blot.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 1:100-1:200Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
Specificity：Human Rat
Isotype：IgG
Calc Applications Abbrev：IF WB
Calc Crossreactivity：Hu Rt
Immunogen：Peptide corresponding to the sequence around aa187-191 (V-D-S-V-A) derived from Human MKK3.
