84-4349-62　AZI1 100ul　307407

特徴

• Component of centriolar satellites, which build a complex and dynamic network required to regulate cilia/flagellum formation.
• During normal and stress-induced ciliogenesis, its non-ubiquitinated form is rapidly displaced from centriolar satellites and recruited to centrosome/basal bodies in a p38 MAPK-dependent manner.
• In contrast, in proliferating cells, MIB1-mediated ubiquitination induces its sequestration within centriolar satellites, precluding untimely cilia formation initiation.
• Acts also as a negative regulator for the trafficking from the centriolar satellite BBSome protein complex to the cilia.
• Plays a role in sperm flagellar formation; may be involved in the regulation of intraflagellar transport (IFT) and/or intramanchette (IMT) trafficking, which are important for axoneme extension and/or cargo delivery to the nascent sperm tail.
• Required for optimal cell proliferation and cell cycle progression; may play a role in the regulation of genome stability and centriole duplication in non-ciliogenic cells.Kikuno R., DNA Res.
• 6:197-205(1999).
• Andersen J.S., Nature 426:570-574(2003).
• Olsen J.V., Cell 127:635-648(2006).
• Applications:Suitable for use in Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:3000Immunohistochemistry: 1:50-1:100Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.