Component of the post-replicative DNA mismatch repair system (MMR).
Forms two different heterodimers: MutS alpha (MSH2-MSH6 heterodimer) and MutS beta (MSH2-MSH3 heterodimer) which binds to DNA mismatches thereby initiating DNA repair.
When bound, heterodimers bend the DNA helix and shields ~20 base pairs.
MutS alpha recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA.
MutS beta recognizes larger insertion-deletion loops up to 13 nucleotides long.
After mismatch binding, MutS alpha or beta forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis.
ATP binding and hydrolysis play a pivotal role in mismatch repair functions.
The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone.
This transition is crucial for mismatch repair.
MutS alpha may also play a role in DNA homologous recombination repair.
In melanocytes may modulate both UV-B-induced cell cycle regulation and apoptosis.Fishel R., Cell 75:1027-1038(1993).
Wang Y., Genes Dev.
14:927-939(2000).
Wang Y., Proc.
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100:15387-15392(2003).
Applications:Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 1:100-1:500Immunohistochemistry: 1:50-1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
Specificity：Human Mouse
Isotype：IgG
Calc Applications Abbrev：IF IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to internal of human MSH2.
