Transcriptional regulator that binds to DNA as a dimer or as a tetramer, but not as a monomer.
Binds to G-doublets in an A/T-rich environment; the preferred motif is a tandem repeat of 5'-.
ATTGGTTA-3' combined with a 5'-TTATTA-3' box.
Binds to nucleosomes By similarity.
Binds to chromatin and interacts selectively with histone H3 that is not methylated at 'Lys-4', not phosphorylated at 'Thr-3' and not methylated at 'Arg-2'.
Functions as a sensor of histone H3 modifications that are important for the epigenetic regulation of gene expression.
Functions as a transcriptional activator and promotes the expression of otherwise tissue-specific self-antigens in the thymus, which is important for self tolerance and the avoidance of autoimmune reactions.Nagamine K., Nat.
Genet.
17:393-398(1997).
Aaltonen J., Nat.
Genet.
17:399-403(1997).
Hattori M., Nature 405:311-319(2000).
Applications:Suitable for use in Immunofluorescence and Western Blot.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 1:100-1:500Western Blot: 1:500-1:3000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
Specificity：Human Mouse
Isotype：IgG
Calc Applications Abbrev：IF WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to internal of human AIRE.
