84-4347-40　PIAS2 100ul　329576

特徴

• Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor.
• Plays a crucial role as a transcriptional coregulator in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway.
• The effects of this transcriptional coregulation, transactivation or silencing may vary depending upon the biological context and the PIAS2 isoform studied However, it seems to be mostly involved in gene silencing.
• Binds to sumoylated ELK1 and enhances its transcriptional activity by preventing recruitment of HDAC2 by ELK1, thus reversing SUMO-mediated repression of ELK1 transactivation activity.
• Isoform PIAS2-beta, but not isoform PIAS2-alpha, promotes MDM2 sumoylation.
• Isoform PIAS2-alpha promotes PARK7 sumoylation.
• Isoform PIAS2-beta promotes NCOA2 sumoylation more efficiently than isoform PIAS2-alpha.
• Isoform PIAS2-alpha sumoylates PML at'Lys-65' and 'Lys-160'.Christina-Maria Hecker, J.
• Biol.
• Chem., Jun 2006; 281: 16117 - 16127.
• Daria Donati, J.
• Immunol., SepHeitham Hassoun, 2006; 177: 3035 - 3044.
• Heitham Hassoun, Am J Physiol Renal Physiol, Feb 2007; 10.1152.
• David J.
• Sharkey, Mol.
• Hum.
• Reprod., Jul 2007;
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:3000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.