84-4346-57　MAP3K8 (Ab-400) 100ul　323129

特徴

• Required for lipopolysaccharide (LPS)-induced, TLR4-mediated activation of the MAPK/ERK pathway in macrophages, thus being critical for production of the proinflammatory cytokine TNF-alpha (TNF) during immune responses.
• Involved in the regulation of T-helper cell differentiation and IFNG expression in T-cells.
• Involved in mediating host resistance to bacterial infection through negative regulation of type I interferon (IFN) production.
• In vitro, activates MAPK/ERK pathway in response to IL1 in an IRAK1-independent manner, leading to up-regulation of IL8 and CCL4.
• Transduces CD40 and TNFRSF1A signals that activate ERK in B-cells and macrophages, and thus may play a role in the regulation of immunoglobulin production.
• May also play a role in the transduction of TNF signals that activate JNK and NF-kappa-B in some cell types.
• In adipocytes, activates MAPK/ERK pathway in an IKBKB-dependent manner in response to IL1B and TNF, but not insulin, leading to induction of lipolysis.
• Plays a role in the cell cycle.
• Isoform 1 shows some transforming activity, although it is much weaker than that of the activated oncogenic variant.
• Applications:Suitable for use in Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:3000Immunohistochemistry: 1:50-1:100Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.