Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest, activation of DNA repair and apoptosis in response to the presence of DNA double-strand breaks.
May also negatively regulate cell cycle progression during unperturbed cell cycles.
Following activation, phosphorylates numerous effectors preferentially at the consensus sequence [L-X-R-X-X-S/T].
Regulates cell cycle checkpoint arrest through phosphorylation of CDC25A, CDC25B and CDC25C, inhibiting their activity.
Inhibition of CDC25 phosphatase activity leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression.
May also phosphorylate NEK6 which is involved in G2/M cell cycle arrest.
Regulates DNA repair through phosphorylation of BRCA2, enhancing the association of RAD51 with chromatin which promotes DNA repair by homologous recombination.
Also stimulates the transcription of genes involved in DNA repair (including BRCA2) through the phosphorylation and activation of the transcription factor FOXM1.
Regulates apoptosis through the phosphorylation of p53/TP53, MDM4 and PML.
Phosphorylation of p53/TP53 at 'Ser-20' by CHEK2 may alleviate inhibition by MDM2, leading to accumulation of active p53/TP53.
Phosphorylation of MDM4 may also reduce degradation of p53/TP53.
Also controls the transcription of pro-apoptotic genes through phosphorylation of the transcription factor E2F1.
Tumor suppressor, it may also have a DNA damage-independent function in mitotic spindle assembly by phosphorylating BRCA1.
Its absence may be a cause of the chromosomal instability observed in some cancer cells.Bhoumik A, et al.
(2005) Mol Cell; 18(5): 577-87.
Gorgoulis VG, et al.
(2005) Nature; 434(7035): 907-13.
Falck J, et al.
(2005) Nature; 434(7033): 605-11.
Jin ZH, et al.
(2005) Oncogene; 24(12): 1973-81.
Li J, et al.
(2005) J Biol Chem; 280(12): 12041-50.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:3000Immunohistochemistry: 1:50-1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
Specificity：Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthesized non-phosphopeptide derived from human Chk2 around the phosphorylation site of threonine 68.
