84-4346-30　RFA2 (Ab-21) 100ul　332519

特徴

• As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress.
• It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism.
• Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage.
• In the cellular response to DNA damage the RPA complex controls DNA repair and DNA damage checkpoint activation.
• It is required for the recruitment of the DNA double-strand break repair factors RAD52 and RAD51 to chromatin in response to DNA damage.
• Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair.
• Plays also a role in base excision repair (BER) probably through interaction with UNG.
• Through RFWD3 may activate CHEK1 and play a role in replication checkpoint control.
• Also recruits SMARCAL1/HARP which is involved in replication fork restart to sites of DNA damage.
• May also play a role in telomere maintenance.Erdile L.F., J.
• Biol.
• Chem.
• 265:3177-3182(1990).
• Gregory S.G., Nature 441:315-321(2006).
• Niu H., J.
• Biol.
• Chem.
• 272:12634-12641(1997).
• Cho J.M., Nucleic Acids Res.
• 28:3478-3485(2000)
• Applications:Suitable for use in Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:3000Immunohistochemistry: 1:50-1:100Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.