Rb operates in the midst of the cell cycle clock apparatus.
Its main role is to act as a signal transducer connecting the cell cycle clock with the transcriptional machinery (Ref.1).
It plays an important role in the Rb/ E2F pathway in cell proliferation, cell fate determination, and cancer (Ref.2).
Cellular senescence is a stable form of cell cycle arrest that limits proliferation of damaged cells and act as a natural barrier to cancer progression.
A distinct heterochromatic structure that accumulates in senescent human fibroblasts, designated as SAHF (Senescence-Associated Heterochromatic Foci).
SAHF formation coincides with recruitment of heterochromatin proteins and the Rb protein to E2F-responsive promoters and is associated with the stable repression of E2F target genes.
Both SAHF formation and the silencing of E2F target genes depended on the integrity of the Rb pathway and do not occur in reversibly arrested cells (Ref.3).
Rb activates transcription of the c-Jun gene through the SP1-binding site within the c-Jun promoter Roesch A, et al.
(2005) Mod Pathol.
18(4): 565-572.
Chadee DN, et al.
(2004) Nat Cell Biol.
6(8): 770-776.
Knudsen ES, et al.
(1997) Mol Cell Biol.
17(10): 5771-5783.
Knudsen ES, et al.
(1996) J Biol Chem.
271(14): 8313-8320.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
Specificity：Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Peptide corresponding to the sequence around aa805-809 (Y-I-S-P-L) derived from human Rb.
