Myc proto-oncogene encodes nuclear DNA-binding phosphoproteins that are involved in the regulation of gene expression and DNA replication during cell growth and differentiation.
Myc encodes a protein of 65kD which is expressed in almost all normal and transformed cells.
The expression correlates with the proliferation state of the cells.
Transcription is repressed in quiescent or terminally differentiated cells.
Expression of Myc is generally induced after mitogenic stimulation of cells or serum induction.
Myc therefore is an important positive regulator of cell growth and proliferation.
Myc has been demonstrated also to be a potent inducer of apoptosis when expressed in the absence of serum or growth factors.
Apoptosis may serve also as a protective mechanism to prevent tumorigenicity elicited by deregulated Myc expression.
Sequences of the Myc oncogene have been highly conserved throughout evolution, from Drosophila to vertebrates Jin Z, et al.
(2004) J Biol Chem.
279(38): 40209-40219.
Welcker M, et al.
(2004) Proc Natl Acad Sci U S A.
101(24): 9085-9090.
Baudino T A, et al.
(2001) Mol Cell Biol.
21: 691-702.
Blackwood E M, et al.
(1991) Science.
251:1211-1217.
Henriksson M, et al.
(1996) Adv Cancer Res.
68: 109-182.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
Specificity：Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Peptide corresponding to the sequence around aa56-60 (L-P-T-P-P) derived from human Myc.
