Acts as negative regulator of T-cell receptor (TCR) signaling by direct dephosphorylation of the Src family kinases LCK and FYN, ITAMs of the TCRz/CD3 complex, as well as ZAP70, VAV, VCP and other key signaling molecules.
Associates with and probably dephosphorylates CBL.
Dephosphorylates LCK at its activating 'Tyr-394' residue.
Dephosphorylates ZAP70 at its activating 'Tyr-493' residue.
Dephosphorylates the immune system activator SKAP2.Cohen S., Blood 93:2013-2024(1999).
Liu T., Submitted (JUL-1998) to the EMBL/GenBank/DDBJ databases.
Wang S., Submitted (JAN-2010) to the EMBL/GenBank/DDBJ databases.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:3000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
Specificity：Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to Internal of human PTPN22.
