84-4341-82　PLK3 100ul　329996

特徴

• Serine/threonine-protein kinase involved in cell cycle regulation, response to stress and Golgi disassembly.
• Polo-like kinases act by binding and phosphorylating proteins are that already phosphorylated on a specific motif recognized by the POLO box domains.
• Phosphorylates ATF2, BCL2L1, CDC25A, CDC25C, CHEK2, HIF1A, JUN, p53/TP53, p73/TP73, PTEN, TOP2A and VRK1.
• Involved in cell cycle regulation: required for entry into S phase and cytokinesis.
• Phosphorylates BCL2L1, leading to regulate the G2 checkpoint and progression to cytokinesis during mitosis.
• Plays a key role in response to stress: rapidly activated upon stress stimulation, such as ionizing radiation, reactive oxygen species (ROS), hyperosmotic stress, UV irradiation and hypoxia.
• Involved in DNA damage response and G1/S transition checkpoint by phosphorylating CDC25A, p53/TP53 and p73/TP73.
• Phosphorylates p53/TP53 in response to reactive oxygen species (ROS), thereby promoting p53/TP53-mediated apoptosis.
• Phosphorylates CHEK2 in response to DNA damage, promoting the G2/M transition checkpoint.
• Phosphorylates the transcription factor p73/TP73 in response to DNA damage, leading to inhibit p73/TP73-mediated transcriptional activation and pro-apoptotic functions.
• Phosphorylates HIF1A and JUN is response to hypoxia.
• Phosphorylates ATF2 following hyperosmotic stress in corneal epithelium.
• Also involved in Golgi disassembly during the cell cycle: part of a MEK1/MAP2K1-dependent pathway that induces Golgi fragmentation during mitosis by mediating phosphorylation of VRK1.
• May participate in endomitotic cell cycle, a form of mitosis in which both karyokinesis and cytokinesis are interrupted and is a hallmark of megakaryocyte differentiation, via its interaction with CIB1.Holtrich U., Oncogene 19:4832-4839(2000).
• Gregory S.G., Nature 441:315-321(2006).
• Mural R.J., Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:3000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.