84-4340-91　PNN 100ul　330057

特徴

• Transcriptional activator binding to the E-box 1 core sequence of the E-cadherin promoter gene; the core-binding sequence is 5'CAGGTG-3'.
• Capable of reversing CTBP1-mediated transcription repression.
• Auxiliary component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs.
• The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism.
• Participates in the regulation of alternative pre-mRNA splicing.
• Associates to spliced mRNA within 60 nt upstream of the 5'-splice sites.
• Component of the PSAP complex which binds RNA in a sequence-independent manner and is proposed to be recruited to the EJC prior to or during the splicing process and to regulate specific excision of introns in specific transcription subsets.
• Involved in the establishment and maintenance of epithelia cell-cell adhesion.
• Potential tumor suppressor for renal cell carcinoma.Ouyang P., J.
• Cell Biol.
• 135:1027-1042(1996).
• Degen W.G.J., Biochim.
• Biophys.
• Acta 1444-384-394(1999).
• Shi Y., Oncogene 19:289-297(2000).
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:3000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.