Carboxypeptidases have functions ranging from digestion of food to selective biosynthesis of neuroendocrine peptides.
Members of the A/B subfamily of carboxypeptidases, such as CPA5, contain an ~90-amino acid pro region that assists in the folding of the active carboxypeptidase domain.
Cleavage of the pro region activates the enzyme (Wei et al., 2002 [PubMed 11836249]).[supplied by OMIM, Mar 2008]Maestrini E., Mol.
Psychiatry 7:289-301(2002).
Moore G.E., J.
Med.
Genet.
40:249-256(2003).
Sugano S., Nat.
Genet.
36:40-45(2004)
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:3000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
Specificity：Human
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to internal of human CPA5.
