Serine/threonine-protein kinase that plays an essential role in the regulation of actin filament dynamics.
Acts downstream of several Rho family GTPase signal transduction pathways.
Activated by upstream kinases including ROCK1, PAK1 and PAK4, which phosphorylate LIMK1 on a threonine residue located in its activation loop.
LIMK1 subsequently phosphorylates and inactivates the actin binding/depolymerizing factors cofilin-1/CFL1, cofilin-2/CFL2 and destrin/DSTN, thereby preventing the cleavage of filamentous actin (F-actin), and stabilizing the actin cytoskeleton.
In this way LIMK1 regulates several actin-dependent biological processes including cell motility, cell cycle progression, and differentiation.
Phosphorylates TPPP on serine residues, thereby promoting microtubule disassembly.
Stimulates axonal outgrowth and may be involved in brain development.
Isoform 3 has a dominant negative effect on actin cytoskeletal changes.
Required for atypical chemokine receptor ACKR2-induced phosphorylation of cofilin (CFL1).Mizuno K., Oncogene 9:1605-1612(1994).
Frangiskakis J.M., Cell 86:59-69(1996).
Osborne L.R., Genomics 36:328-336(1996).
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:3000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
Specificity：Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to internal of human LIMK1.
