84-4332-40　Syk 100ul　336222

特徴

• Non-receptor tyrosine kinase which mediates signal transduction downstream of a variety of transmembrane receptors including classical immunoreceptors like the B-cell receptor (BCR).
• Regulates several biological processes including innate and adaptive immunity, cell adhesion, osteoclast maturation, platelet activation and vascular development.
• Assembles into signaling complexes with activated receptors at the plasma membrane via interaction between its SH2 domains and the receptor tyrosine-phosphorylated ITAM domains.
• The association with the receptor can also be indirect and mediated by adapter proteins containing ITAM or partial hemITAM domains.
• The phosphorylation of the ITAM domains is generally mediated by SRC subfamily kinases upon engagement of the receptor.
• More rarely signal transduction via SYK could be ITAM-independent.
• Direct downstream effectors phosphorylated by SYK include VAV1, PLCG1, PI-3-kinase, LCP2 and BLNK.
• Initially identified as essential in B-cell receptor (BCR) signaling, it is necessary for the maturation of B-cells most probably at the pro-B to pre-B transition.
• Activated upon BCR engagement, it phosphorylates and activates BLNK an adapter linking the activated BCR to downstream signaling adapters and effectors.
• It also phosphorylates and activates PLCG1 and the PKC signaling pathway.
• It also phosphorylates BTK and regulates its activity in B-cell antigen receptor (BCR)-coupled signaling.
• Beside its function downstream of BCR plays also a role in T-cell receptor signaling.
• Plays also a crucial role in the innate immune response to fungal, bacterial and viral pathogens.
• It is for instance activated by the membrane lectin CLEC7A.
• Upon stimulation by fungal proteins, CLEC7A together with SYK activates immune cells inducing the production of ROS.
• Also activates the inflammasome and NF-kappa-B-mediated transcription of chemokines and cytokines in presence of pathogens.
• Regulates neutrophil degranulation and phagocytosis through activation of the MAPK signaling cascade.
• Also mediates the activation of dendritic cells by cell necrosis stimuli.
• Also involved in mast cells activation.
• Also functions downstream of receptors mediating cell adhesion.
• Relays for instance, integrin-mediated neutrophils and macrophages activation and P-selectin receptor/SELPG-mediated recruitment of leukocytes to inflammatory loci.
• Plays also a role in non-immune processes.
• It is for instance involved in vascular development where it may regulate blood and lymphatic vascular separation.
• It is also required for osteoclast development and function.
• Functions in the activation of platelets by collagen, mediating PLCG2 phosphorylation and activation.
• May be coupled to the collagen receptor by the ITAM domain-containing FCER1G.
• Also activated by the membrane lectin CLEC1B that is required for activation of platelets by PDPN/podoplanin.
• Involved in platelet adhesion being activated by ITGB3 engaged by fibrinogen.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:3000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.