Non-receptor tyrosine-protein kinase that plays an essential role in the selection and maturation of developing T-cells in the thymus and in the function of mature T-cells.
Plays a key role in T-cell antigen receptor (TCR)-linked signal transduction pathways.
Constitutively associated with the cytoplasmic portions of the CD4 and CD8 surface receptors.
Association of the TCR with a peptide antigen-bound MHC complex facilitates the interaction of CD4 and CD8 with MHC class II and class I molecules, respectively, thereby recruiting the associated LCK protein to the vicinity of the TCR/CD3 complex.
LCK then phosphorylates tyrosines residues within the immunoreceptor tyrosine-based activation motifs (ITAM) of the cytoplasmic tails of the TCR-gamma chains and CD3 subunits, initiating the TCR/CD3 signaling pathway.
Once stimulated, the TCR recruits the tyrosine kinase ZAP70, that becomes phosphorylated and activated by LCK.
Following this, a large number of signaling molecules are recruited, ultimately leading to lymphokine production.
LCK also contributes to signaling by other receptor molecules.
Associates directly with the cytoplasmic tail of CD2, which leads to hyperphosphorylation and activation of LCK.
Also plays a role in the IL2 receptor-linked signaling pathway that controls the T-cell proliferative response.
Binding of IL2 to its receptor results in increased activity of LCK.
Is expressed at all stages of thymocyte development and is required for the regulation of maturation events that are governed by both pre-TCR and mature a beta TCR.
Phosphorylates other substrates including RUNX3, the microtubule-associated protein MAPT, RHOH or TYROBP.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
Specificity：Human Mouse
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo
Immunogen：Peptide corresponding to the sequence around aa392-396(N-E-Y-T-A) derived from Human Lck.
