GRASP (GRP1-associated scaffold protein, tamalin) is a 395 amino acid proteinencoded by the human gene GRASP .
GRASP is a scaffold protein that com-prises multiple protein-interacting domains, including a 95kD postsynaptic density protein (PSD-95)/discs-large/ZO-1 (PDZ) domain, a leucine-zipperregion and a carboxyl-terminal PDZ-binding motif.
GRASP is involved with intracellular trafficking and contributes to the macromolecular organization of group 1 metabotropic glutamate receptors (mGluRs) at synapses.
GRASP forms a heteromer composed of GRASP , PSCD2 and at least one mGluR-1.
It also interacts with PSCD3, mGluR-2, mGluR-3 and mGluR-5.
GRASP is highly expressed in brain and has lower levels of expression in lung, heart, embryo, kidney and ovary.
Kitano, J., Kimura, K., Yamazaki, Y., Soda, T., Shigemoto, R., Nakajima, Y.and Nakanishi, S.
2002.
Tamalin, J.
Neurosci.
22: 280-1289.
Hall, B.S., Gabernet-Castello, C., Voak, A., Goulding, D., Natesan, S.K.
and Field, M.C.
2006.
J.
Biol.
Chem.
281: 27600-27612.
Sugi, T., Oyama, T., Muto, T., Nakanishi, S., Morikawa, K.
and Jingami, H.
2007.
Crystal structures of autoinhibitory PDZ domain of Tamalin EMBO J.26: 2192-2205.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
Specificity：Human Mouse Rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Peptide corresponding to the sequence around aa814-818 (Q-E-I-V-R ) derived from rat GRASP.
