84-4330-37　Tyrosine Hydroxylase 100ul　338671

特徴

• Tyrosine hydroxylase (TH) catalyzes the rate-limiting step in the synthesis of the neurotransmitter dopamine and other catecholamines.
• TH functions as a tetramer, with each subunit composed of a regulatory and catalytic domain, and exists in several different isoforms .
• This enzyme is required for embryonic development since TH knockout mice die before or at birth .
• Levels of transcription, translation and posttranslational modification regulate TH activity.
• The amino-terminal regulatory domain contains three serine residues: Ser9, Ser31 and Ser40.
• Phosphorylation at Ser40 by PKA positively regulates the catalytic activity of TH .
• Phosphorylation at Ser31 by CDK5 also increases the catalytic activity of TH through stabilization of TH protein levels.
• Applications:Suitable for use in ELISA and Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.