Factor inhibiting HIF-1 (FIH-1) exists as a homodimer and binds to HIF-1alpha.
Specifically, FIH-1 operates as an asparaginyl hydroxylase.
It catalyzes the hydroxylation of the beta-carbon of Asparagine residue 803 within the carboxy terminal transactivation domain of HIF-1alpha.
This hydroxylation event blocks the association of HIF-1alpha with co-activators.
FIH-1 also binds to von Hippel-Lindau (VHL) tumor suppressor protein, which represses transcriptional ac-tivity of HIF-1alpha.
In transiently transfected human osteosarcoma cells, FIH-1 localizes to the cytoplasm.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:2000-1:10000Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:300Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide, 40% glycerol.
