84-4307-16　LIMK1 50ul　322428

特徴

• There are ~40 known eukaryotic LIM proteins, so named for the LIM domains they contain.
• LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers.
• Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions.
• LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain.
• LIMK1 is a serine/threonine kinase that regulates actin polymerization via phosphorylation and inactivation of the actin binding factor cofilin.
• This protein is ubiquitously expressed during development and plays a role in many cellular processes associated with cytoskeletal structure.
• Applications:Suitable for use in ELISA and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:1000-1:2000Immunohistochemistry: 1:25-1:100Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.