Stable cohesion between sister chromatids before anaphase and their timely separation during anaphase are critical for chromosome inheritance.
In vertebrates, sister chromatid cohesion is released in 2 steps via distinct mechanisms.
The first step involves phosphorylation of STAG1 or STAG2 in the cohesin complex.
The second step involves cleavage of the cohesin subunit SCC1 by ESPL1, or separase, which initiates the final separation of sister chromatids.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:1000-1:2000Western Blot: 1:200-1:1000Immunohistochemistry: 1:25-1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide, 40% glycerol.
