NIMA was originally shown in Aspergillus nidulans to be necessary for entry into mitosis.
NIMA-related mammalian proteins have since been identified as Nek1, Nek2, Nek3 and Nek4 (also designated STK2 or NRK2).
High expression of Nek1 is seen in male and female germ cell lines of mouse.
Nek2 is the closest known mammalian relative to NIMA.
Like NIMA, Nek2 expression peaks at the G2 to M phase transition.
Nek3 is a predominantly cytoplasmic enzyme that was detectable in all organs studied.
Levels of Nek3 seem to remain unchanged throughout the cell cycle, but appear to be elevated in G0-arrested, quiescent fibroblasts.
In developing testicular germ cells, differential patterns of expression were seen for Nek1, Nek2 and Nek4, indicating possible overlapping, but non-identical functions.
Applications:Suitable for use in ELISA and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:2000-1:5000Immunohistochemistry: 1:25-1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide, 40% glycerol.
