Sodium/calcium exchanger proteins are integral membrane proteins primarily seen in cardiac cells.
In cardiac myocytes, the concentration of Ca2+ alternates between low levels during relaxation and high levels during contraction.
The Na+/Ca2+ exchanger 1 (NCX1) protein mediates Ca2+ extrusion from cardiac cells during relaxation.
Four NCX1 isoforms (NCX1.1, NCX1.3, NCX1.7, and NCX1.10) result from alternate splicing.
NCX1 mRNA is present at high levels in the heart, with lower levels present in the brain.
NCX2 is most abundantly expressed in brain, in contrast the the broader distribution of NCX1, which is also expressed in heart, kidney, lung, smooth and skeletal muscle.
The difference in expression for the transporter subtypes is believed to reflect differences in their functional roles.
Regulation mechanisms for these exchanger proteins have not been fully characterized.
Applications:Suitable for use in ELISA and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:1000-1:2000Immunohistochemistry: 1:25-1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide, 40% glycerol.
