84-4282-30　Lambda Light chain 100ul　322114

特徴

• Antibody producing cells of the immune system require multiple rearrangements of immunoglobulin (antibody, Ig) genes.
• Immunoglobulins are four-chain, Y-shaped, monomeric structures of two identical heavy chains and two identical light chains held together through interchain disulfide bonds.
• Immunoglobulins in vertebrates help to remove non-self molecules or cells (antigens) by recognizing and binding to the antigen and carrying out effector functions that activate the immune system.
• Variable genetic combinations of the five heavy chain classes (M, D, G, E and A) and the two light chain isotypes, Kappa and Lambda, confer the role of an antibody.
• The variable region genes encoding immunoglobulin Kappa and Lambda chains are assembled from three DNA segments, the V, C and J genes.
• Kappa and Lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region.
• The ratio of Kappa to Lambda is 70:30 , the vast majority of which is bound to heavy-chain in immunoglobulin.
• Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:2000-1:5000Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.