Layilin is a recently cloned 55kD membrane-binding partner for talin (Borowsky and Hynes, 1998) that is widely expressed in different cell types and tissue extracts.
It is found in peripheral ruffles of spreading cells and is recruited to membrane ruffles in cells induced to migrate in in vitro wounding experiments.
Layilin colocalizes with talin in ruffles and binds to talin's 50kD head domain.
Other parts of talin (the 220kD tail fragment) can bind beta-integrin cytoplasmic tails, vinculin, and F-actin and hence form an integrin-cytoskeleton linkage at sites of cell-substratum contact.
Layilin's extracellular domain is homologous with the carbohydrate-recognition domains (CRD) of C-type lectins.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:1000-1:2000Western Blot: 1:200-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide, 40% glycerol.
Specificity：Recognizes human LAYN. Species Crossreactivity: mouse
