Forkhead/winged helix transcription factor, Foxj3, was expressed in embryonic and adult skeletal muscle.
The 100-kb, 13-exon mouse Foxj3 gene on chromosome 4 encodes a 623aa (aa) protein from an mRNA of at least 4.8 kb (Human FOXJ3: Chr 1, 627aa, 5.3-kb mRNA).
During the stages of mouse development investigated Foxj3 is expressed in neuroectoderm, in neural crest, and in many structures derived from neural crest cells, such as facioacoustic, trigeminal, and dorsal root ganglia.
Stripes of expression appear at E10.5 in the location of myotomes and expand ventrally in a pattern similar to the developing body wall musculature.
Developing limbs have a complex pattern of Foxj3 expression that at E12.5 colocalizes with the condensed mesenchyme of the skeletal primordia.
Applications:Suitable for use in ELISA, Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:1000-1:5000Western Blot: 1:1000-1:3000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide, 40% glycerol.
Specificity：Recognizes human FOXJ3. Species Crossreactivity: mouse and rat
