84-4259-18　RAN 50ul　331948

特徴

• RAN (ras-related nuclear protein) is a small GTP binding protein belonging to the RAS superfamily that is essential for the translocation of RNA and proteins through the nuclear pore complex.
• The RAN protein is also involved in control of DNA synthesis and cell cycle progression.
• Nuclear localization of RAN requires the presence of regulator of chromosome condensation 1 (RCC1).
• Mutations in RAN disrupt DNA synthesis.
• Because of its many functions, it is likely that RAN interacts with several other proteins.
• RAN regulates formation and organization of the microtubule network independently of its role in the nucleus-cytosol exchange of macromolecules.
• RAN could be a key signaling molecule regulating microtubule polymerization during mitosis.
• RCC1 generates a high local concentration of RAN-GTP around chromatin which, in turn, induces the local nucleation of microtubules.
• RAN is an androgen receptor (AR) coactivator that binds differentially with different lengths of polyglutamine within the androgen receptor.
• Polyglutamine repeat expansion in the AR is linked to Kennedy's disease (X-linked spinal and bulbar muscular atrophy).
• RAN coactivation of the AR diminishes with polyglutamine expansion within the AR, and this weak coactivation may lead to partial androgen insensitivity during the development of Kennedy's disease.
• Applications:Suitable for use in ELISA and Western Blot.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:1000-1:5000Western Blot: 1:200-1:1000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• ; IHC: 1:25-1:100