The RAB3D cDNA encodes a 24-kD protein as determined by in vitro translation.
The deduced 219-amino acid sequence shows highest homology to the RAB3 subfamily of low molecular mass GTP-binding proteins.
Northern blot analysis of various human hematopoietic cell lines showed a single transcript of 4.1 kb present at variable levels in each cell line, with highest expression in granulocytic lines.
RAB3D was also highly expressed in YJ and HL-60 cells in response to retinoic acid treatment.
Applications:Suitable for use in ELISA, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:1000-1:10,000Immunohistochemistry: 1:25-1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:50ul
Host:rabbit
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide, 40% glycerol.
Specificity:Recognizes human RAB3D. Species Crossreactivity: mouse and rat