The DnaJ family is one of the largest of all the chaperone families and has evolved with diverse cellular localization and functions.
The presence of a J domain defines a protein as a member of the DnaJ family.
DnaJ heat shock induced proteins are from the bacterium Escherichia coli and are under the control of the htpR regulatory protein.
The DnaJ proteins play a critical role in the HSP 70 chaperone machine by interacting with HSP 70 to stimulate ATP hydrolysis.
DnaJ proteins are important mediators of proteolysis and are involved in the regulation of protein degradation, exocytosis and endocytosis.
MCJ (methylation-controlled J protein), also known as HSD18, DNAJD1 or DNAJC15, is a 150aa ubiquitously expressed single-pass membrane protein containing one J domain.
Localizing to the golgi apparatus and only present in vertebrates, MCJ may be associated with increased chemotherapeutic resistance in ovarian cancer by inducing expression of the Mdr drug transporter and preventing intracellular drug accumulation.
Applications:Suitable for use in ELISA and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:2000-1:5000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide, 40% glycerol.
