The epidermal growth factor (EGF) repeat-containing proteins constitute an expanding family of proteins that are involved in several cellular activities, such as blood coagulation, fibrinolysis, cell adhesion and neural and vertebrate development.
CRELD2 (cysteine-rich with EGF-like domains 2) is a 353aa protein that is ubiquitously expressed and contains two FU domains and two EGF-like domains.
Localized to the endoplasmic reticulum and secreted into the cell, CRELD2 interacts with AChR 4, possibly regulating its transport.
Human CRELD2 shares 69% amino acid identity with its mouse counterpart, suggesting a conserved role between species.
Multiple isoforms of CRELD2 exist due to alternative splicing events.
The gene encoding CRELD2 maps to human chromosome 22, which houses over 500 genes and is the second smallest human chromosome.
Mutations in several of the genes that map to chromosome 22 are involved in the development of Phelan-McDermid syndrome, Neurofibromatosis type 2, autism and schizophrenia.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:2000-1:5000Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide, 40% glycerol.
