CARF (calcium-response factor), also known as ALS2CR8 (amyotrophic lateral sclerosis 2 chromosomal region candidate gene 8 protein) or NYD-SP24, is a 725aa nuclear protein.
Expressed in a wide variety of tissues with highest expression in the hippocampus, CARF is thought to be a transcription factor that associates with the p53 tumor suppression pathway.
CARF cooperates, colocalizes and is co-regulated with ARF, an ADP-ribosylation factor, and, through this interaction, helps to mediate ARF-p53-induced apoptotic signaling.
This apoptotic pathway is implicated in cell cycle control, proper cellular development, response to DNA damage and the aging process, suggesting that CARF participates in various events throughout the cell.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:1000-1:2000Western Blot: 1:200-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide, 40% glycerol.
