SIMPL, also known as IRAK1BP1 (interleukin-1 receptor-associated kinase 1-binding protein 1), is a 260aa protein belonging to the IRAK1BP1 family.
In addition to containing an intrinsically disordered region which associates with HSPA1 and HSPA8, SIMPL also interacts directly with IRAK1 and promotes RELA transcriptional activity.
SIMPL is a major component of the IRAK1-dependent TNFRSF1A signaling pathway in which TNF-alpha induces NF-kappa-B gene transcription, leading to an inhibition of proinflammatory cytokines.
Inactivation of SIMPL inhibits tumor necrosis factor receptor type I-induced NF-kappaB activity.
Localizing to the nucleus and cytoplasm, SIMPL requires the phosphorylation of Ser-56 and/or Ser-62 to be fully active.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:2000-1:5000Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide, 40% glycerol.
