Dok3 was tyrosine phosphorylated by Src family members Lck, Fyn, and Lyn.
Immunoprecipitation studies showed that Dok3 bound inhibitors SHIP and Csk but did not bind RasGAP.
Dok3 binding to SHIP occurred via the SH2 domain.
Dok3 also bound Csk via the Csk SH2 domain with possible involvement of the Csk SH3 domain as well.DOK proteins are enzymatically inert adaptor or scaffolding proteins.
They provide a docking platform for the assembly of multimolecular signaling complexes.
DOK3 is a negative regulator of JNK signaling in B-cells through interaction with INPP5D/SHIP1.
May modulate ABL1 function
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:1000-1:2000Western Blot: 1:200-1:1000Immunohistochemistry: 1:25-1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide, 40% glycerol.
