Cytochrome c oxidase is the terminal enzyme of the electron transfer chain in aerobic bacteria as well as in the mitochondria of plants and animals.
Bacterial cytochrome c oxidases are composed of three different subunits and include two hemes a and two copper atoms as prosthetic groups.
The enzyme from eukaryotes is more complex and includes three subunits encoded on mitochondrial DNA, which are the homologues of the subunits of the bacterial enzyme, and in addition contains a number of subunits encoded in the nucleus.
It is generally agreed that the mitochondrially coded subunits with their associated prosthetic groups are the functional core of the enzyme.
The role of the nuclear coded subunits in cytochrome c oxidase function remains a matter of conjecture.
cytochrome c oxidase subunit VIb polypeptide 2 Connects the two COX monomers into the physiological dimeric form.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:2000-1:5000Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide, 40% glycerol.
