Calpains are ubiquitous, well-conserved family of calcium-dependent, cysteine proteases.
The calpain proteins are heterodimers consisting of an invariant small subunit and variable large subunits.
The large subunit possesses a cysteine protease domain, and both subunits possess calcium-binding domains.
Calpains have been implicated in neurodegenerative processes, as their activation can be triggered by calcium influx and oxidative stress.
The function of the protein encoded by this gene is not known.
An orthologue has been found in mouse but it seems to diverge from other family members.
The mouse orthologue is thought to be calcium independent with protease activity.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:2000-1:5000Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide, 40% glycerol.
