NFS1 (nitrogen fixation 1), also known as NIFS or IscS (cysteine desulfurase), is a member of the class V pyridoxal-phosphate-dependent aminotransferase family.
It localizes to the cytoplasm or mitochondrion depending on which form is generated based on cytosolic pH.
Highest expression levels of NFS1 are found in heart and skeletal muscle.
Lower levels of expression are also found in liver, brain and pancreas.
NFS1 is responsible for catalyzing the removal of sulfur from cysteine to form alanine, thereby supplying the inorganic sulfur for iron-sulfur (Fe-S) clusters.
Fe-S clusters function as essential cofactors in a wide variety of events, including facilitation of electron transfer processes in oxidative phosphorylation, catalysis of enzymatic reactions in aconitase and dehydratases and maintenance of structural integrity in the DNA repair enzyme endonuclease III.
Applications:Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.3, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes human NFS1. Species Crossreactivity: mouse and rat
