84-4055-09　［取扱停止］NDRG1 100ul　325368

特徴

• N-myc downstream-regulated gene 1 (NDRG1), also termed Cap43, Drg1, RTP/rit42, and Proxy-1, is a member of the NDRG family, which is composed of four members (NDRG1-4) that function in growth, differentiation, and cell survival .
• NDRG1 is ubiquitously expressed and highly responsive to a variety of stress signals including DNA damage, hypoxia , and elevated levels of nickel and calcium.
• Expression of NDRG1 is elevated in N-myc defective mice and is negatively regulated by N- and c-myc.
• During DNA damage, NDRG1 is induced in a p53-dependent fashion and is necessary for p53-mediated apoptosis.
• Research studies have shown that NDRG1 may also play a role in cancer progression by promoting differentiation, inhibiting growth, and modulating metastasis and angiogenesis.
• Nonsense mutation of the NDRG1 gene has been shown to cause hereditary motor and sensory neuropathy-Lom (HMSNL), which is supported by studies demonstrating the role of NDRG1 in maintaining myelin sheaths and axonal survival.
• NDRG1 is up-regulated during mast cell maturation and its deletion leads to attenuated allergic responses.
• Both NDRG1 and NDRG2 are substrates of SGK1, although the precise physiological role of SGK1-mediated phosphorylation is not known.
• NDRG1 is phosphorylated by SGK1 at Thr328, Ser330, Thr346, Thr356, and Thr366.
• Phosphorylation by SGK1 primes NDRG1 for phosphorylation by GSK-3.
• Applications:Suitable for use in Western Blot, Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.