IL-7 plays a key role in lymphopoiesis and lymphoid homeostasis.
Stromal and epithelial cells within the bone marrow and thymus produce IL-7.
The primary targets of IL-7 are T cells, B cells, and dendritic cells.
IL-7 is crucial for T cell development, the importance of which is underscored by the lack of T cells in both mice and humans that are deficient in IL-7/IL-7R signaling.
While IL-7 appears to be required for B cell development in mice, the role of IL-7 in human B cell development is unclear.
In addition to its effects on T cell lymphopoiesis, IL-7 promotes the maintenance and survival of naï,ve and memory alphabeta T cells, as well as gammaδ T cells.
The IL-7 receptor is a heterodimer of the common gamma chain, gammac, and the IL-7-specific IL-7Ralpha.
IL-7 activates PI3K/Akt, Jak1/2, and Stat1, 3, and 5.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.3, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human IL7. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant protein corresponding to human IL7
