Lymphotoxin-alpha (LT-alpha), also known as TNF–beta, is a member of the TNF superfamily of proteins.
NK cells, T cells, B cells, and lymphoid tissue-inducer cells express LT-alpha.
LT-alpha can be secreted as a soluble homotrimer or form membrane bound heterotrimers with lymphotoxin-beta (LT alpha1beta2 or LT alpha2beta1) which can be cleaved from the cell surface by matrix metalloproteases.
Soluble LT-alpha binds to and signals through TNFR1/TNFR2, activating the canonical NF-kappaB pathway.
In contrast, LT alpha1beta2 heterodimers bind to the LTbetaR receptor and activate the noncanonical NF-kappaB pathway.
As a result, LT-alpha and TNF-alpha have overlapping functions.
Soluble LT-alpha and LT alpha1beta2 play key roles in lymphangiogenesis.
The LT alpha1beta2/LTbetaR axis is essential for the development of lymphoid tissue.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.3, 0.02% sodium azide, 50% glycerol.
Specificity：Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant protein corresponding to human LTA
