Heterotrimeric guanine nucleotide-binding proteins (G proteins) consist of alpha, beta and gamma subunits and mediate the effects of hormones, neurotransmitters, chemokines and sensory stimuli.
To date, over 20 known Galpha subunits have been classified into four families, Galpha(s), Galpha(i/o), Galpha(q) and Galpha(12), based on structural and functional similarities.
Phosphorylation of Tyr356 of Galpha(q)/Galpha(11) is essential for activation of the G protein, since phenylalanine substitution for Tyr356 changes the interaction of Galpha with receptors and abolishes ligand-induced IP3 formation.Galpha(z) stands out from other G proteins because it lacks an ADP-ribosylation consensus site for pertussis toxin, giving it a possible role in signal transduction pathways resistant to the toxin, such as phospholipase C.
Galpha(z) is phosphorylated and activated by protein kinase C (PKC) at Ser27.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.3, 0.02% sodium azide, 50% glycerol.
Specificity：Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant protein corresponding to human GNAZ
