IL-2 is a T cell stimulatory cytokine best known for inducing T cell proliferation and NK cell proliferation and activation.
IL-2 also promotes peripheral development of Treg cells.
Conversely, IL-2 is involved in activation induced cell death (AICD) that is observed post T cell expansion by increasing levels of Fas on CD4+ T cells.
The effects of IL-2 are mediated through a trimeric receptor complex consisting of IL-2Ralpha, IL-2Rbeta and the common gamma chain, gammac.
IL-2 Ralpha binds exclusively to IL-2 with low affinity and increases binding affinity of the whole receptor complex including IL-2Rbeta and gammac subunits.
IL-2Rbeta is also used by IL-15.
The common gammac is used by other cytokines including IL-4, IL-7, IL-9, IL-15, and IL-21.
Binding of IL-2 initiates signaling cascades involving Jak1, Jak3, Stat5 and the PI3K/Akt pathways.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.3, 0.02% sodium azide, 50% glycerol.
Specificity：Human
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to human IL2
